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KMID : 0613820070170010070
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Journal of Life Science
2007 Volume.17 No. 1 p.70 ~ p.75
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Isolation and characterization of a marine bacterium Thalassomonas sp. SL-5 producing β-agarase
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Lee Dong-Geun
Kim Nam-Young
Jang Min-Kyung
Lee Ok-Hee
Lee Sang-Hyeon
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Abstract
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A novel agar-degrading bacterium SL-5 was isolated from seashore of Homigot at Kyung-Buk province, and cultured in marine broth 2216 media. The bacterium SL-5 was identified as Thalassomonas genus by 16S rDNA sequencing with 96% identity. Growth rate was faster at 27℃ than at 37℃ and agarase was produced as growth-related. The optimum pH of the enzyme activity was 7.0 and the optimum temperature for the reaction was 40℃. Although the enzyme had no thermostability, the enzyme activity was remained over 80% at 60℃. The enzyme hydrolyzed neoagarohexaose to yield neoagarobiose as the main product, indicating that the enzyme is β-agarase. Thus, the enzyme would be useful for the industrial production of neoagarobiose
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KEYWORD
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β-agarase, marine, Thalassomonas sp.
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